# 02.折线图绘制

### 清除当前环境中的变量

``````rm(list=ls())
``````

### 设置工作目录

``````setwd("C:/Users/Dell/Desktop/R_Plots/02lineplot/")
``````

## base plot函数绘制普通折线图

``````# 读取示例数据
# 查看数据
##   x   y1   y2   y3
## 1 1  7.0 -0.2 -0.9
## 2 2  6.9  0.8  0.6
## 3 3  9.5  5.7  3.5
## 4 4 14.5 11.3  8.4
## 5 5 18.2 17.0 13.5
## 6 6 21.5 22.0 17.0

dim(data)
## [1] 12  4

attach(data)
plot(x, y1, type = "b", pch = 15, lty = 1, col = "red", xlab = "时间", ylab = "反应强度", ylim = c(-1,30))
lines(x, y2, type = "b", pch = 16, lty = 2, col = "blue")
lines(x, y3, type = "b", pch = 17, lty = 3, col = "purple")
legend("topleft", inset = 0.02, title = "样品", c("y1","y2","y3"),
lty = c(1,2,3), pch = c(15,16,17), col = c("red","blue","purple"),
bg = "gray")
detach(data)
``````
image.png

## ggplot2包绘制带误差棒的折线图

``````library(ggplot2)
# 读取示例数据
data2 <- read.table("demo2_lineplot.txt", header = T, row.names = 3, sep="\t", check.names = F)
data2\$Stage <- factor(data2\$Stage,levels = c("0 min","10 min","20 min","30 min","45 min","60 min","90 min","120 min"))
# 查看数据
##          Stage GeneSymbol Expression Log10(Expression)
## 0min_1   0 min       EGT2   1207.380          3.081844
## 0min_2   0 min       EGT2   1209.970          3.082775
## 0min_3   0 min       EGT2   1089.770          3.037335
## 10min_1 10 min       EGT2    801.201          2.903741
## 10min_2 10 min       EGT2    704.513          2.847889
## 10min_3 10 min       EGT2    597.385          2.776254

#+++++++++++++++++++++++++
# Function to calculate the mean and the standard deviation
# for each group
#+++++++++++++++++++++++++
# data : a data frame
# varname : the name of a column containing the variable
#to be summariezed
# groupnames : vector of column names to be used as
# grouping variables
# 定义函数计算平均值和标准差
data_summary <- function(data, varname, groupnames){
require(plyr)
summary_func <- function(x, col){
c(mean = mean(x[[col]], na.rm=TRUE),
sd = sd(x[[col]], na.rm=TRUE))
}
data_sum <- ddply(data, groupnames, .fun=summary_func, varname)
data_sum <- rename(data_sum, c("mean" = varname))
return(data_sum)
}

data2 <- data_summary(data2, varname="Expression",
groupnames=c("Stage"))
##    Stage Expression        sd
## 1  0 min  1169.0400  68.66205
## 2 10 min   701.0330 101.95255
## 3 20 min   701.8853 196.06879
## 4 30 min   682.8983 186.25895
## 5 45 min   788.3107  72.94692
## 6 60 min   749.8397 261.03729

# Standard deviation of the mean
ggplot(data2, aes(x=Stage, y=Expression, group=1, color=Stage)) +
geom_errorbar(aes(ymin=Expression-sd, ymax=Expression+sd), width=.1) +
geom_line() + geom_point()+
scale_color_brewer(palette="Paired")+theme_bw()
``````
image.png

# ggplot2包绘制聚类趋势折线图

``````# 读取示例数据
# 查看数据
##     Gene   Stage1    Stage2    Stage3    Stage4    Stage5     Stage6
## 1 Gene01 1.155864 0.9021231 -1.085794 -1.265632 0.2614499 0.03198916
## 2 Gene02 1.145877 0.8973008 -1.095785 -1.266790 0.2721922 0.04720437
## 3 Gene03 1.125560 0.8994570 -1.124195 -1.255849 0.2777603 0.07726622
## 4 Gene04 1.114566 0.8681610 -1.115066 -1.283721 0.3224320 0.09362759
## 5 Gene05 1.129178 0.8700930 -1.097511 -1.288186 0.3142035 0.07222275
## 6 Gene06 1.138877 0.8809866 -1.092992 -1.281036 0.2962881 0.05787733
##    Group
## 1 group1
## 2 group1
## 3 group1
## 4 group1
## 5 group1
## 6 group1

library(reshape2)
# 将宽数据格式转换为长数据格式
data3 = melt(data3)
## Using Gene, Group as id variables

##     Gene  Group variable    value
## 1 Gene01 group1   Stage1 1.155864
## 2 Gene02 group1   Stage1 1.145877
## 3 Gene03 group1   Stage1 1.125560
## 4 Gene04 group1   Stage1 1.114566
## 5 Gene05 group1   Stage1 1.129178
## 6 Gene06 group1   Stage1 1.138877

names(data3) <- c("Gene","Group","Stage","Expression")

ggplot(data3,aes(x=Stage, y=Expression, group=Gene)) + geom_line(color="gray90",size=0.8) +
geom_hline(yintercept =0,linetype=2) +
stat_summary(aes(group=1),fun.y=mean, geom="line", size=1.2, color="#c51b7d") +
facet_wrap(.~Group) + theme_bw() +
theme(panel.grid.major = element_blank(), panel.grid.minor = element_blank(),
axis.text = element_text(size=8, face = "bold"),
strip.text = element_text(size = 10, face = "bold"))
``````
image.png
``````sessionInfo()
## R version 3.6.0 (2019-04-26)
## Platform: x86_64-w64-mingw32/x64 (64-bit)
## Running under: Windows 10 x64 (build 18363)
##
## Matrix products: default
##
## locale:
## [1] LC_COLLATE=Chinese (Simplified)_China.936
## [2] LC_CTYPE=Chinese (Simplified)_China.936
## [3] LC_MONETARY=Chinese (Simplified)_China.936
## [4] LC_NUMERIC=C
## [5] LC_TIME=Chinese (Simplified)_China.936
##
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base
##
## other attached packages:
## [1] reshape2_1.4.3 plyr_1.8.4     ggplot2_3.2.0
##
## loaded via a namespace (and not attached):
##  [1] Rcpp_1.0.1         knitr_1.23         magrittr_1.5
##  [4] tidyselect_0.2.5   munsell_0.5.0      colorspace_1.4-1
##  [7] R6_2.4.0           rlang_0.4.0        stringr_1.4.0
## [10] dplyr_0.8.3        tools_3.6.0        grid_3.6.0
## [13] gtable_0.3.0       xfun_0.8           withr_2.1.2
## [16] htmltools_0.3.6    yaml_2.2.0         lazyeval_0.2.2
## [19] digest_0.6.20      assertthat_0.2.1   tibble_2.1.3
## [22] crayon_1.3.4       RColorBrewer_1.1-2 purrr_0.3.2
## [25] glue_1.3.1         evaluate_0.14      rmarkdown_1.13
## [28] labeling_0.3       stringi_1.4.3      compiler_3.6.0
## [31] pillar_1.4.2       scales_1.0.0       pkgconfig_2.0.2
``````